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Typhoon FLA 7000

eagle-i ID


Resource Type

  1. Imager


  1. Resource Description
    " Typhoon FLA 7000 is a fast and versatile laser scanner for biomolecular imaging applications including sensitive and quantitative measurements of radioisotopic labels, chemifluorescent Western blots, and single fluorescence (visible excitation), as well as documentation of colorimetric stains (e.g., Coomassie blue and silver-stained gels). Fast, simple, and sensitive imaging for filmless autoradiography, fluorescence, chemifluorescence, and gel documentation for multiple users Use multiple imaging modes at any time with no system modifications, reducing effort for occasional users Fast scanning without loss of sensitivity reduces queues and improves quantitation 16-bit images at up to 25 μm pixel resolution give precise quantitation in gels and blots Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 32P, 33P, 35S and other sources of ionizing radiation. Simultaneous imaging of up to eight 10 x 8 cm blots facilitates comparisons, reducing workload and waiting time Typhoon FLA 7000 IP provides dedicated fast, simple, and sensitive imaging for radioisotope detection ( 3H, 14C, 32P, 33P, 35S, and other sources of ionizing radiation). Typhoon FLA 7000 is a nonconfocal variable mode laser scanner offering high speed and resolution for precise quantitation of proteins, nucleic acids, and other biomolecules. Using predefined methods, the system can image a sample measuring 24 x 40 cm in 2.5 minutes, offering high throughput for the multiuser lab environment. The system provides several imaging modes: fluorescence and chemifluorescence, filmless autoradiography, and digitization of stained samples (e.g. Coomassie blue and silver stain). Applications include the detection of proteins stained with Deep Purple, SYPRO Ruby or SYPRO Red, chemifluorescent Western blot imaging with ECL Plus, and DNA imaging by SYBR Green or Cy5. The system provides a linear signal response over five orders of magnitude. This, together with digitization of the images with up to 16-bit resolution, provides a suitable basis for the precise quantitation of proteins, DNA, and other suitably labeled molecules in complex samples. The system employs cost-effective and space-saving diode lasers. Four computer-controlled filters are included. Filters are easily installed by the user without tools for attaining optimal imaging conditions. Two stages for fluorescence and phosphorimaging are included. Supported formats include agarose and polyacrylamide gels, membranes, and radioisotope-labeled samples. The optional Multi Stage enables imaging of polyacrylamide gels with glass plates. The TP plug-in accompanies the Multi Stage and provides a means to image up to three microplates. All stages are easily removed from the system for cleaning. Typhoon FLA 7000 IP is a fast laser scanner for biomolecular imaging for sensitive and quantitative measurement of radioisotopic labeled samples. It offers high-speed performance for sensitive detection and precise quantitation of radioisotopic signals detected by storage phosphor screen technology. This is a simple configuration of Typhoon FLA 7000 without any fluorescence functionalities.Technical specifications for phosphorimaging are identical to those of Typhoon FLA 7000."
  2. Contact
    Porrata-Doria, Tirtsa
  3. Manufacturer
    General Electric Company
  4. Model Number
    FLA 7000
  5. Website(s)
  6. Location
    Molecular and Genomics Core
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016