eagle-i Ponce School of Medicine and Health SciencesPonce School of Medicine and Health Sciences
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Molecular and Genomics Core

Director: Noel, Richard, Ph.D.


Molecular biology is a field with a tremendous capacity for advancing our scientific understanding of disease and human health. Our Core facility provides its users with a centralized source of expertise as well as access to sophisticated instrumentation. This strong combination of knowledge and equipment helps to increase the productivity of the scientists at PSM so that they may more quickly make discoveries that can be translated to the clinical level and thus have a measurable impact on improving health in the US and worldwide.




  • Bio-Rad PTC-200 thermal cycler ( Thermal cycler )

    12-well gradient function; accessory 384-well module.

  • BioRad iCycler IQ Real-Time PCR Detection System ( Real-time PCR machine )

    Four color real time fluorescence detection.

  • BioTek Synergy HT Multi-Mode Microplate Reader ( Multimode microplate reader )

    Dual injector; detection modes include: luminescence, fluorescence and visible light.

  • Cellometer K2 Image Cytometer ( Image cytometer )

    "The Cellometer K2 utilizes bright field imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported."

  • Eco qPCR system ( Real-time PCR machine )

    "The Eco Real-Time PCR System is an easy to use qPCR system for both new and experienced Real-Time PCR users. The Eco platform supports all chemistries and Real-Time PCR applications, including: absolute quantification, relative quantification, allelic discrimination, and genotyping."

  • Eppendorf AG realplex2 real-time PCR machine ( Real-time PCR machine )

    Two color real time fluorescence detection.

  • Experion automated electrophoresis station ( Gel tank )

    "The Experion automated electrophoresis station performs all of the steps of gel-based electrophoresis in one compact, durable unit. It automates analysis by combining electrophoresis, staining, destaining, band detection, and imaging into a single, 30 min step. It's an electrophoresis cell, power supply, and imager built into a single device and is designed for easy, precise operation"

  • gentleMACS Dissociator ( Microdissection instrument )

    "The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation of tissues into single-cell suspensions or thorough homogenates." Programmable tissue disruption for culture and molecular analyses.

  • LI-COR 4300 DNA analyzer ( DNA sequencer )

  • MJ Research DNA engine dyad ( Thermal cycler )

    Three independent PCR modules of 96-gradients and 48/48 samples. Independent block control, 12-well gradient function.

  • Typhoon FLA 7000 ( Imager )

    " Typhoon FLA 7000 is a fast and versatile laser scanner for biomolecular imaging applications including sensitive and quantitative measurements of radioisotopic labels, chemifluorescent Western blots, and single fluorescence (visible excitation), as well as documentation of colorimetric stains (e.g., Coomassie blue and silver-stained gels).
    Fast, simple, and sensitive imaging for filmless autoradiography, fluorescence, chemifluorescence, and gel documentation for multiple users
    Use multiple imaging modes at any time with no system modifications, reducing effort for occasional users
    Fast scanning without loss of sensitivity reduces queues and improves quantitation
    16-bit images at up to 25 μm pixel resolution give precise quantitation in gels and blots
    Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 32P, 33P, 35S and other sources of ionizing radiation.
    Simultaneous imaging of up to eight 10 x 8 cm blots facilitates comparisons, reducing workload and waiting time
    Typhoon FLA 7000 IP provides dedicated fast, simple, and sensitive imaging for radioisotope detection ( 3H, 14C, 32P, 33P, 35S, and other sources of ionizing radiation).

    Typhoon FLA 7000 is a nonconfocal variable mode laser scanner offering high speed and resolution for precise quantitation of proteins, nucleic acids, and other biomolecules. Using predefined methods, the system can image a sample measuring 24 x 40 cm in 2.5 minutes, offering high throughput for the multiuser lab environment. The system provides several imaging modes: fluorescence and chemifluorescence, filmless autoradiography, and digitization of stained samples (e.g. Coomassie blue and silver stain). Applications include the detection of proteins stained with Deep Purple, SYPRO Ruby or SYPRO Red, chemifluorescent Western blot imaging with ECL Plus, and DNA imaging by SYBR Green or Cy5.

    The system provides a linear signal response over five orders of magnitude. This, together with digitization of the images with up to 16-bit resolution, provides a suitable basis for the precise quantitation of proteins, DNA, and other suitably labeled molecules in complex samples.

    The system employs cost-effective and space-saving diode lasers. Four computer-controlled filters are included. Filters are easily installed by the user without tools for attaining optimal imaging conditions.

    Two stages for fluorescence and phosphorimaging are included. Supported formats include agarose and polyacrylamide gels, membranes, and radioisotope-labeled samples. The optional Multi Stage enables imaging of polyacrylamide gels with glass plates. The TP plug-in accompanies the Multi Stage and provides a means to image up to three microplates. All stages are easily removed from the system for cleaning.

    Typhoon FLA 7000 IP is a fast laser scanner for biomolecular imaging for sensitive and quantitative measurement of radioisotopic labeled samples. It offers high-speed performance for sensitive detection and precise quantitation of radioisotopic signals detected by storage phosphor screen technology. This is a simple configuration of Typhoon FLA 7000 without any fluorescence functionalities.Technical specifications for phosphorimaging are identical to those of Typhoon FLA 7000."

  • UVP EpiChemi II darkroom ( Imager )

    Detection modes: chemiluminescence, visible light
    Light sources: uv-short, uv-long, visible.



  • FCS4 Express Cytometry ( Software )

    "FCS Express 4 Image Cytometry Software leverages De Novo Software's flow cytometry analysis and reporting tools to create a new environment for image cytometry analysis. These fully interactive tools for reviewing images, dot plots and single cell galleries enable you to tame multi-parametric data sets, improving the image anaylsis workflow and your ability to manage results."

  • Gen5 Data Analysis Software ( Software )

    "Gen5 is a unique combination of power and ease-of-use that drives productivity and saves time. Use Gen5 to control BioTek’s readers and export data, or as a fully integrated processing tool."

Last updated: 2017-04-17T12:09:46.795-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016